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5723 2564
Published in Volume 2, Issue 4 -

The iterative informed consent model for the feedback of incidental findings in human health research using WGS procedures

Felicitas Holzer

Languages: English

DOI: 10.17160/josha.2.4.45

Facing the upcoming application possibilities of genomic sequencing in human health research, to determine the appropriate ethical scope of genetic counselling for research participants becomes a most important challenge. In this thesis, an informed consent model for the disclosure of incidental findings in research using whole genome sequencing or whole exome sequencing will be presented. The design of an informed consent model is always based on a general informed consent theory. Thus, informed consent models can be defined as application of an ideal informed consent theory, containing general ethical principles, to a particular context.


2524 1548
Published in Volume 2, Issue 3 -

JOSHA - Table of Contents Volume 2 Issue 3

Felicitas Holzer

Languages: English

DOI: 10.17160/josha.2.3.42

This issue of the JOSHA Journal contains a wide range of interesting articles in the fields of human research, arts, history and education. This time the special topic will focus on mentoring in the academic context from a Latin American Perspective. “International Cooperation and Mentoring: An Academic Obligation?” is discussed by Carlos Conzález Correa from the University of Concepción, as well as by Cristina Arranz from the University of Buenos Aires, offering a complementary Argentine view point. Furthermore, we present a paper “Gesang ist Dasein, Todeskonzeption und Dichtung als Seinsstiftung im Spätwerk Rilkes insbesondere den Sonnetten an Orpheus“ in the field of art which addresses the hermaneutic method within Rainer Maria Rilke’s oevre.


2556 2094

Analysis of the Dopaminergic Systems Development and Function by Targeted Genome Editing

Kristine Østevold

Languages: English

Dopamine is a prominent modulatory neurotransmitter affecting a vast array of neural circuits, however, dopamine has also been shown to be an important regulator of neurogenesis both in the developing embryo and in the adult mammalian brain. A more thorough understanding of dopamine and its role in neurogenesis will be pivotal to exploit endogenously produced neural precursors for cell replacement in neurodegenerative disorders such as Parkinson’s Disease. Two genes important for dopaminergic function are tyrosine hydroxylase (th), the rate limiting enzyme in the synthesis of catecholamines, and the re-uptake carrier dopamine transporter (dat / slc6a3). Using TALENs technology, mutations were induced in the th and dat genes of zebrafish embryos.


2180 1495
Published in Volume 2, Issue 4 -

Hepatocellular alterations and dysregulation of oncogenic pathways in the liver of transgenic mice overexpressing growth hormone

Thomas Freund

Languages: English

DOI: 10.17160/josha.2.4.40

The use of growth hormone has risen in the last years. Apart from its interactions in growth and metabolism, its use has been approved in different clinical conditions which do not necessarily implicate the lack of GH. Furthermore, GH is used to prevent attributes of aging and to gain better athletic condition, without being approved for these cases. In this context, it should be clear that the use of GH is not free from side effects. A serious side effect of the use or the abuse of GH could be the development of tumors. The excess of GH is associated with the development of liver tumors in mice. Possible mechanisms for GH promoting this type of cancer imply its effect on cellular proliferation and the modulation of signaling components involved in a large range of processes i.e. inflammation.


2465 2310
Published in Volume 2, Issue 4 -

THE IMPACT OF DIFFUSION ON BIOCHEMICAL ASSAY KINETICS IN 3D-HYDROGEL MICROARRAYS

Mostafa Mahmoud

Languages: English

DOI: 10.17160/josha.2.4.39

Microarrays are powerful and versatile analytical tools. They can address the complex diagnosis of various diseases and disorders in a fast, reliable and simple manner. They consist of very small test sites in form of spots on which various biochemical assays can be performed simultaneously. In the past few years, this technology has developed a lot with the implementation of novel surface chemistries, detection techniques, and different assay formats. 3D-hydrogel microarrays were developed using unique immobilization techniques based on hydrophilic polymer networks. The 3D polymer network attaches and immobilizes the capture molecules onto the surface in form of a spot with molecules immobilized both on the surface and inside the gel matrix. This retains the molecule’s natural confirmation and structure and enables higher immobilization efficiency, which allows for higher sensitivity.


2619 3838
Published in Volume 2, Issue 3 -

AMERICA COMES FROM FREIBURG: Cartographer Waldseemüller from Freiburg was the first to show the New World as a Continent in 1507 and named it AMERICA

Rudolf-Werner Dreier

Languages: English

DOI: 10.17160/josha.2.3.37

”America“ is a word that is now part of our everyday language. We speak of ”America” and the ”Americans” rather than of the ”United States”. But where does this name come from? Very few people are aware of the fact that it was actually the work of a cartographer from Freiburg, who was the first to show the New World as a Continent in 1507 and named it AMERICA, which led to the popularization of this name throughout the world.


2098 1870
Published in Volume 2, Issue 3 -

Growth hormone (GH) modulation of Epidermal growth factor (EGF) signalling in human mammary epithelial cells

Verena Wölker

Languages: English

DOI: 10.17160/josha.2.3.36

This study demonstrates crosstalk between Growth Hormone (GH) and Epidermal Growth Factor (EGF) in two different breast cancer cell lines. Long-term modification of cell viability and the ERK1/2 and AKT pathways was only detected in MDA-231 cells. By simultaneous treatment with GH and EGF, the interaction between GH and EGF in MCF-7 cells could be transiently abrogated by GH-pre-treatment. Co-treatment with GH and EGF diminished cell viability in MCF-7 and MDA-231 cells. Furthermore, a direct influence between GH and EGF signaling resulted in less ERK phosphorylation in MCF-7 cells, whereas in MDA-231 cells less AKT phosphorylation could be observed. EGFR-down regulation due to GH is a potential reason but keeps to be elucidated.


3194 1960
Published in Volume 2, Issue 3 -

JOSHA - Table of Contents Volume 2 Issue 2

Roland Mertelsmann

Languages: English

DOI: 10.17160/josha.2.3.35

JOSHA – Journal of Science Humanities and Arts Table of Contents Vol 2, No 2 This new issue of the JOSHA Journal contains again a number of interesting articles covering the broad field of human research, discovery and creativity and, as a special topic, “International Cooperation and Mentoring” in academic institutions. The field of Arts is addressed by two wonderful papers, addressing art in the northern provinces of the Roman Empire as well as the creativity of a living artist. Aspects of Philosophy, Ethics and Law are studied in the paper on the philosopher Spinoza and his cultural and religious environment. In Life Sciences a fundamental process of the effect of human growth factor application to epidermal growth factor biology is studied.


2822 1857
Published in Volume 2, Issue 3 -

International Cooperation and Mentoring: An Academic Obligation? University of Concepción - CHILE

Carlos González Correa

Languages: English

DOI: 10.17160/josha.2.3.34

Concepción is the capital city of the Bio-Bio Region. ➢ It is located in the middle of Chile, close to the Pacific Ocean, and 500 kilometers south of Santiago. ➢ The Region is identified with the hydrographic basin of the Bio-Bio River, one of the most important fluvial streams in the country. Chile hold 64 Universities - Group 1: Include 25 members called the Rectors Council Group. - Group 2: Composed by 39 private Universities of different owners and orientations ➢ Also has 48 Professional Institutes and 117 Technical Training Centers In 1967, the Ministry of Eductation created an autonomous office called “National commission for scientific research and technology”, CONICYT. The two major objectives were the training of human capital and the promoting, developing, and disseminating scientific and technological research. The aim is to Contribute to Chile's economic, social, and culture development.


3129 1803
Published in Volume 2, Issue 3 -

Current trends in Community-acquired methicillin-resistant Staphylococcus aureus in Argentina

Marta Mollerach

Languages: English

DOI: 10.17160/josha.2.3.33

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) infections have become a major concern worldwide. Different from hospital-acquired MRSA, CA-MRSA usually is staphylococcal cassette chromosome (SCCmec) type IV, carries genes for Panton-Valentine leukocidin (PVL) and is susceptible to several non ß-lactam antibiotics. The epidemic of CA- MRSA is evolving. A specific clone that is initially propagating and causing CA-MRSA infections can be displaced by a more successful one. In Argentina, previous studies have identified sequence type 5 (ST5), SCCmec IV, spa type 311 as the predominant CA-MRSA clone causing infections and colonizing children.