3D printed scaffold to monitor hanging drops on inverted microscopes

Lukas Peintner

Affiliation: Institute of Molecular Medicine and Cell Research, Albert-Ludwigs-University Freiburg, Freiburg, Germany

Keywords: 3D Cell culture, spheroids, microscopy, additive printing, 3D printing, sterile, hanging drop

Categories: Life Sciences

DOI: 10.17160/josha.6.2.531

Languages: English

Growing cells in 3D cell culture became a major field in cell biology over the last decade. Cells growing in a dimensionally unrestricted space spontaneously form organoids or spheroids that express cellular behavior different from cells growing on a plane surface. However, monitoring three dimensional spheroids on standard inverted microscopes proved difficult since hanging drops are often outside the focus range. Here I present the design and production of a cover slip tray by conventional additive printing (3D printing) able to fit into a single well of a six-well plate to enable the observation of hanging drop spheroids using inverted microscopes.

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