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In this experiment by Nina Raad, the MCF-7 breast cancer cell line was used because it is a commonly used breast cancer cell line that has been propagated for many years by multiple groups. It proves to be a suitable model cell line for breast cancer investigations worldwide, including those regarding anticancer drugs. Cells were loaded in the 8 well ship and treated with different concentrations of ATP - (20,30,40,50,70,100 micromolar) . It took from 3 seconds to 120 seconds to obtain the signal, depending on the concentration. The effect was reversible in MCF-7 with lower concentrations of ATP, however, with higher concentrations it was irreversible in some cases due to the toxic effect of ATP on cells; in lower concentrations it takes more time to reach the toxic level.

Kosovo Modernism counts a significant number of good architecture examples, easily compered with the construction activity of the socialist modernism in the region. Yet, compered to the architecture of the same period of other countries of Yugoslavia, it remains a ‘grey area’. It is less analysed and documented compared with the Byzantine and Islamic Architecture, whose conquest counts a significant influence on building urban and architectonic identity of the country. Modernism in Kosovo came in the name of prosperity and progress. But this meant that a number of traditional buildings and old city cores were erased following the maxim ‘destroy the old, place the new’, to make place for new modernist structures. Major cities of Kosovo witnessed major transformations. Also, these transformations were not led by the local authorities, but were mostly imposed from outside.

Dealing with grief is hard no matter the person, length of illness, or the time period. Death during COVID19 is not the same and the impact of the loss is more than just the death of a loved one but the way we mourn moving forward. The Covid Diaries – The Last Days of My Normal In this book Monica Hickson — freelance writer, instructional designer and life-long learner aims to help people understand that which can’t be understood. Monica´s website www.monicahickson.com

Conventional cell culture systems are primarily two dimensional (2D). They are low cost and robust, and ensure homogeneous cell proliferation due to equal access to nutrients. However, they do not accurately represent in vivo conditions, with only horizontal spreading, in case of adherent cells. Three dimensional (3D) culture systems are now becoming increasingly popular since the conditions in these systems are similar to those in vivo, due to the presence of a matrix. 3D systems also make it easier to image and track individual cells over long periods of time, especially suspension cells. Continuous long term live cell imaging using a 3D culture system can aid in the detection of minute morphological changes in cells which can serve as indicators of complex cellular processes.

The Together Project is a series of photo essays inspiring us to see humankind in a different light by honoring our cultural differences as well as the understanding that we’re all cut from the same cloth; we’re members of humankind. The Together Project is here to inspire you to learn about others and to ignite a sense of curiosity and compassion towards those who live in different cultures from our own. In this photo essay, Johanna explores Varanasi, India shares her experience as well as the customs that take place in the enchanting city.

Patient health is the primary issue for any health professional. Therefore, we, as students in the nursing branch and as future nurses , want through this research work to expand our knowledge and at the same time to offer before you our experience with terminal patients who were part of palliative care. Palliative care is specialized medical care for people living with a serious illness. This type of care is focused on relief from the symptoms and stress of a serious illness. Palliative care teams specialize in treating people suffering from the symptoms and stress of serious illnesses such as cancer, congestive heart failure (CHF), chronic obstructive pulmonary disease (COPD), kidney disease, Alzheimer’s, Parkinson’s, Amyotrophic Lateral Sclerosis (ALS) and many more. Palliative care can be helpful at any stage of illness and is best provided from the point of diagnosis.

Dear JOSHA readers, In this bi-monthly issue we present you with a diverse selection of articles. We would also like to remind you that you can support us with a small donation. http://josha-journal.org/en/donate Visit our social media #joshajournal and get to know us from the Instagram gallery and the Facebook wall. Remember that you can comment on the articles and also provide your star ranking.

"We get little sunlight, especially in the winter months. However, vitamin D deficiency is not as widespread as supplement manufacturers would lead us to believe. According to statistics from the Robert Koch Institute, only two percent of adults suffer from vitamin D deficiency that requires treatment.“

This thesis employs doctrinal legal analysis methods by using laws, regulations and legal theories, to evaluate the current protections of intellectual property rights for foreign investors in mainland China. In this thesis, it compares the current Chinese laws and regulations on intellectual property protections with its international Fair and Equitable Treatment principles, previous laws, and Phase 1 Trade Agreement. It concludes that Phase 1 Trade Agreement has been implemented mostly in the current Chinese laws, which also have been improved fundamentally to protect foreign investors’ interests and rights. However, given the backgrounds on rule of law in China, this thesis also recognises the formations of Chinese laws are different from those perceived by the Western societies. Therefore, even though China has strive to cohere the standards of international investment law, its laws are still relatively lacking in preciseness and predictability, clarity and effective enforcement.

The Together Project is a series of photo essays inspiring us to see humankind in a different light by simultaneously honoring our differences and highlighting that we are so much more alike than we think we are. The Together Project is here to inspire you to have meaningful conversations, connect with others, to live life curiously, and to believe in one another. In this photo essay, Johanna explores Thabarwa Meditation Center located in Thanlyin, Myanmar and shares her experience as well as the customs that take place there. The author holds the copyright to the pictures of this article.